Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRADFIQFGAMIHGVGGTTDGWRHPDVDPSASTNIEFYMKKAQTAEKGLFSFIFIADGLFISEKSIPHFLNRFEPITILSALASVTKNIGLVGTFS-TS-FTEPFTISRQLMSLDHISGGRAGWNLVTSPQEGAARNHSKSNLPEHTERYEIAQEHLDVVRGLWNSWEHDAFIHNKKTGQFFDQAKLHRLNHKGKYFQVEGPLNIGRSKQGEPVVFQAGSSETGRQFAAKNADAIFTHSNSLEETK-AFYADVKSRAADEGRDPSSVRIFPGISPIVADTEEEAEKKYREFAELIPIENAVTYLARFFDDYDLSVYPLDEPFPDIGDVGKNAFQSTTDRIKREAKARNLTLREVAQEMAFPRTLFIGTPERVASLIETWFNAEAADGFIVGSD---IPGTLDAFVEKVIPILQERGLYRQDYRGGTLRENLGLGIPQHQSVLHSSHH 1RHC Chain:A ((2-330)) -----KTQIGYFASL----------------EQYRPMDALEQAIRAEKVGFDSVWVDDHFHPWYH---DNAQSAQAWAWMGAALQATKKVFISTCITCPIMRYNPAIVAQTFATLRQMYPGRVGVAVGAGEAMNEVPVTGEW--PSVPVRQDMTVEAVKVMRMLWESD--------------------KPVTFKGDYFTLDKAFLYTKPDD-EVPLYFSGMGPKGAKLAGMYGDHLMTVAAAPSTLKNVTIPKFEEGAREAGKDPSKMEHAMLIWYSVDPDYDKAVEALRFWAGCLVPS--M---FKYKV------YDPKE-VQL-------------------H-ANLVHCDTI-----KENYMCATDAEEMIKEIERFKEA-GINHFCLGNSSPDVNFGIDIF-KEVIPAVRD---------------------------------

General information: TITO was launched using: RESULT: Template: 1RHC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1859 -43900 -23.61 -135.91 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -23.61 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.435

(partial model without unconserved sides chains): PDB file : Tito_1RHC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RHC-query.scw PDB file : Tito_Scwrl_1RHC.pdb: