Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKHTFVLFLSLILLVLPGCSAEKSSADTAKKTLTIYSTMSTDSERDTFRKLAAAFEKEHSDIHVSLHF-PGNDYENMMRVRMAAN-DLPDLFDTHG--WGKIRYGEYTADLRDMKW---TQDLDPNLNSILKNKSGKVYAYPINQAKDGLAYNRNILDRYGIAPPETMDDFIKALRTIKEKSKGSIVPFWFAGYDKSSFAQYYDQFATPLLITDPAHNEKKQLINGTFQW-S-KFTYLSEILKQMQKEKLINIDAVTAKKSQLIELMAQNKIAFTMQGGTLGQDVAQIN---PNVKVGIIPTPAIHPGDDPIWIGGERYTLAAWKDSPQLKEAKDFIAFMARPANAKQMAEATSLPSGLTNVKA-DIFYANDY---EYYQDVKVEPYFDRLYLPNG-MWD-VLGTVGQELAADILAPQDISQKLGREYKRLREQSETQGAENNE 3K01 Chain:A ((24-411)) --------------------------VELSGTVTFWDTSNE-AEKATYQALAEGFEKEHPKVDVKYVNVPFGEANAKFKNAAGGNSGAPDVMRTEVAWVADFASIGYLAPLDGTPALDDGSDHLPQAAAS-TRYEGKTYAVPQVIDTLALFYNKELLTKAGVEVPGSVAELKTAAAEIT-E-KTGATGLYLRGDDPYW----FLPYLYG-----EGGDLVDE-KNKTVTVDDEAGVRAYRVIKDLVDSKAAITDA-SDGWNNMQNAFKSGKVAMMVNGPWAIEDVKAGARFKDAGNLGVAPVPAGSAGQG-SP--QGGWNLSVYAGSKNLDASYAFVKYMSSAKVQQQTTEKLSLLPTRTSVYEVPS-VADNEMVKFFKPAVDKA--VERPWIAEGNALFEPIRLQMANVLSGETSPDEAAANTGDAYRKLLKDY---------

General information: TITO was launched using: RESULT: Template: 3K01.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2127 106106 49.89 286.77 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 49.89 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.460

(partial model without unconserved sides chains): PDB file : Tito_3K01.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3K01-query.scw PDB file : Tito_Scwrl_3K01.pdb: