Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELYIITGASKGLGQAIALQALEKGHEVHALSRTKTDV----SHK---KLTQHQIDLINLEEAEQQFETLLSSIDSDRYSGITLINNAGMVTPIKRAGEASLDELQRHYQLNLTAPVLLSQLFTKRFASYSGKKTVVNITSGAAKNPYKGWSAYCSSKAGLDMFTRTFGFEQEDEELPVNMISFSPGVMDTEMQAVIRSSSKKDFHHIERFRKLNETGSLRSPDFIAGTLLSLLEKG--TENGRIYDIKEFL 4ZA2 Chain:A ((10-247)) GKVALITGCDTGLGQGMAIGLAQAGCDIVGVNIVEPKDTIEKVTALGRRFLSLTADMSNVSGHAELVEKAVAE----FGHVDILVNNAGIIRR-EDAIEFSEKNWDDVMNLNIKSVFFMSQTVARQFIKQGKGGKIINIASMLSFQGGIRVPSYTASKSAVMGVTRLMANEWA--KHGINVNAIAPGYMATNNTQQLRAD----EERSKEILDRIPAGRWGLPQDLMGPSVFLASSASDYINGYTIAVD---

General information: TITO was launched using: RESULT: Template: 4ZA2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1186 -25474 -21.48 -111.24 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -21.48 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.468

(partial model without unconserved sides chains): PDB file : Tito_4ZA2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZA2-query.scw PDB file : Tito_Scwrl_4ZA2.pdb: