Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MYCPQCGHQT------DGGNFCEKCGS--PLPGQSGHQHAAQTGAAAKQAAKQFGSFVL---SVLKRPYQECKATG-GEQLISAIITMVLFSLLTPLMFYILFSDGPGSVSFTAIF------LEPTIYFILFLFGLHACIFFALKIAGNQVSFKDSFSRFGAFLIPFTAILILALFFFLLHTDICFTILAVGLIGAFFAIPPAMLSSYQHSYKGKVDFIYSTIVI--YLIICVTFQLIIEHYVKEIFRYMLF--- 2F9I Chain:B ((26-285)) PAGIMTKCPKCKKIMYTKELAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQQKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFILFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGDINLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNDMRQTLSEILKIHQEVTK

General information: TITO was launched using: RESULT: Template: 2F9I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1188 -67194 -56.56 -289.63 target 2D structure prediction score : 0.34 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -56.56 2D Compatibility (Sec. Struct. Predict.) : 0.34 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.116

(partial model without unconserved sides chains): PDB file : Tito_2F9I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F9I-query.scw PDB file : Tito_Scwrl_2F9I.pdb: