Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQEKERILKLVEEGKLTAQEALTLIEKLDSDYKEKEEKITALSVHVHDEEEPFTTAKKESGKPSLGAKLFDWIDSAVKKVKEVDLDLNFGHAYDVQHIFQFKDTDFSSVELQIANGSVNIVPWEDDD---IRAECQAKVYRADSQDA------ARHAFLQHIECEIKGNKFFIRTEKKT----------MKTNVTLYIPQKEYDKIRVKLFNGPVRGEHLHVKE-FSA-KTTNGVLSFSYLTAEKAIAE-TANGQIKLASHSCGTIEAETIN-GLIDLRGKSESIDVQSFNGNIAINVTESDCRSIYAKTT-TGNVELAIPDDLAVKAELKSNLGTLSHELMDVEMLKE-KNDTIQKEMMFTSNQAHDQNITVFSESLTGAIKLKYSQR 3JX8 Chain:A ((4-250)) -------------------------------------------------------------------------------------------------------------DKRIDGNGNPETREIKISDYDEITFVGSADFEYEQSDKAPYLSVTIDENLFDYLVTEVEGGTLKIYPKSIKKGFNNNSYDLRPTVYKIKSNSKELKELNTVGSGSFIISKPTKVNRMEINMAGSGNVELRGPVKGYKLECNMAGSGNIIAKDIQLDNLSCSLASSGEIEVIGTVDRASFN-VAGSGEIKAFDCQARKAECNIASSGEISVYATQILDANIV---GSGEIHYKGDPEISKSIMGSGSINKVK------------------------------

General information: TITO was launched using: RESULT: Template: 3JX8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1327 69288 52.21 312.11 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 52.21 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.393

(partial model without unconserved sides chains): PDB file : Tito_3JX8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3JX8-query.scw PDB file : Tito_Scwrl_3JX8.pdb: