Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKRFSLIMMTGLLFGLTSPAFAAEKTETEAKAPANVAVLLDASGSMAKRIDGVSKFNSAKKEISKFASSLP-EGTQVKMSVFGSEGNNKNSGKVQSCEAIRNVYGFQSF-NEQSFLNSLNTIG-PTGWTPIAKALNEAKSSFDQL----DAKGEKVVYLLTDGEETCGG-NPIKTAKELQKDNITVNVIGFDYKEG---YKGQLNAIAKVGGGE-YFPAYTQKDVEKIFTQQSLMLSK 1MF7 Chain:A ((129-316)) ------------------------------PQEDSDIAFLIDGSGSIIP-----HDFRRMKEFVSTVMEQLKKSKTLFSLMQYSEEF--------------RIHFTFKEFQNNPNPRSLVKPITQLLGRTHTATGIRKVVRELFNITNGARKNAFKILVVITDGEKFGDPLGYEDVIPEADREGVIRYVIGVGDAFRSEKSRQELNTIASKPPRDHVFQVNNFEALKTIQNQLREKI--

General information: TITO was launched using: RESULT: Template: 1MF7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 920 46988 51.07 266.97 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 51.07 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.511

(partial model without unconserved sides chains): PDB file : Tito_1MF7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MF7-query.scw PDB file : Tito_Scwrl_1MF7.pdb: