Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSFMHSKAVIFSFTMAFFLILAACSGKNEADSKDTGWEQIKDKGKIVVATSGTLYPTSYHDTDSGSDKLTGYEVEVVREAAKRLGLKVEFKEMGIDGMLTAVNSGQVDAAANDIDVTKDREEKFAFSTPYKYSYGTAIVRKDDLSGIKTLKDLKGKKAAGAATTVYMEVARKYGA-KEVIYDNATNEQYLKDVANGRTDVILNDYYLQTLALAAFPD-LNITIHPDIKYMPNKQALVMKKSNAALQKKMNEALKEMSKDGSLTKLSKQFFNKADVSKKIDADVQDVDL 2PVU Chain:A ((38-265)) ----------------------------------------GATKKKVVVGTDAAFAPFEYMQK----GKIVGFDVDLLDAVMKAAGLDYELKNIGWDPLFASLQSKEVDMGISGITITDERKQSYDFSDPYFEATQVILVK-QG-SPVKNALDLKGKTIGVQNATTGQEAAEKLFGKGPHIKKFETTVVAIMELLNGGVDAVITDNAVANEYVKNNPNKKLQVIEDPKNFASEYYGMIFPK-NSELKAKVDEALKNVINSGKYTEIYKKWFGKEP--------------

General information: TITO was launched using: RESULT: Template: 2PVU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1201 -96503 -80.35 -427.00 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -80.35 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.586

(partial model without unconserved sides chains): PDB file : Tito_2PVU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PVU-query.scw PDB file : Tito_Scwrl_2PVU.pdb: