Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVMTKKDTNISTQQPLWLQGYIDSPEKQNQLYKKTLKILIFSQIFGGAGLGAGITVGALLAQD----MIGSENVAGIPTALFTFGSAVAALLIGASSQRFGRRAGLAGGFLIGGLGAIGVIIAALINS-------VALLFVSLLIYGAGMASNLQVR-YAGTDLANEKQRATAASMALVSTTLGAVVGPNLVNTMGEFAD-----------SIGVPNLAGPFIMSGAAFIIAGIILLIFLRPDPLFVSTAIANAEKKDDKVQIGGSLKNP-AIDK----KGIMVGAVIMILAQLIMTAIMTMTPVHMGHHGHGLSE----------VGLVIGLHIAAMYLPSPLTGLLVDKFGRTTMAIASGATLLAAGLVAAIAPAD--SLSLLILALVLLGVGWNFGLLTGTALIIDSTHPSLRAKTQGTFDVLLALSGAAGGALSGMVVAHSSYTILSISGAVLSLLLIPVVIWYFRRIQEKA 4J05 Chain:A ((50-495)) ------------------------------------------------DLFIINQVAPMLAQVYFPKTGLPAQRQDLMKAAANIGCVVGQVMFGVLGDSFGRKFVYGKELILIIVATIFQ---MSAPSHWDGNRVLTWITICRVFLGIGIGGDYPMSATVVSDRANIHRRGTLLCFIFANQGWGSFVGSLVTIVTISGFKHRLKSGHTHDVDKAWR---ILIGLSLIPAFGTLYQ-R----------------------------WQEFVAYFSTWNHFRNLLGSMLGWFLVDIAFYGINLNQSVVLAQIGFAGKTGDVYDKLFQLATGNIIVTALGFLPGYYFTLFLIDIVGRKKLQFMGFIMSGLFLAILAGEIDHIGKGPLLACFTFMQFFFNFGANTTTFIVAAELFPTRIRASAHGISAAAGKCGAILSSLVFNQLKAKIGTSAVLWIFFSTCILGF--------------

General information: TITO was launched using: RESULT: Template: 4J05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1725 -238545 -138.29 -703.67 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -138.29 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.298

(partial model without unconserved sides chains): PDB file : Tito_4J05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J05-query.scw PDB file : Tito_Scwrl_4J05.pdb: