Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDLLKSMNRALKYIEENLTNDIDFQEAAKLAFCSEYHFKRMFSFLAGISLSEYIRRRRLTLAAFELKDSNVKVIDIAIKYGYNSPDSFARAFQNLHGINPSKARFNGHSLKAYPRMTFQLTIKGGNEMNYRIEEKEAFRIVGIKKRVPIIFKGINPEIASMWESLDEKAINKLKELSNVAPSGLISASTNFSEGRTEENGELDHYIGAATTK--RCPDNFSRLEVPASTWAVFESIGPFPDTLQEVWGRIYSEWFPSSNYEQIEGPEILW-NEHKDVTSPSFKSEIWIPITKK---- 1D5Y Chain:A ((1-292)) --QAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRSPE--WSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMRYQFWHDFLGNAPT----IPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVMLQGGEYVMFTYEGL-GTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDDRPINLRCELLIPIRRKLAAA

General information: TITO was launched using: RESULT: Template: 1D5Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1339 -94578 -70.63 -336.58 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -70.63 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.383

(partial model without unconserved sides chains): PDB file : Tito_1D5Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1D5Y-query.scw PDB file : Tito_Scwrl_1D5Y.pdb: