Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIDVAAMTRCLKTLSDQTRLIMMRLFLEQEYCVCQLVDMFEMSQPAISQHLRKLKNAGFVNEDR----RGQWRYYSI----NGSCPEFDTLQLILHQIDQEDELLNHIKQKKTQACCQ---------------------------------------------------------------------------------- 2P4W Chain:B ((1-198)) MG---EELNRLLDVLGNETRRRILFLLTKRPYFVSELSRELGVGQKAVLEHLRILEEAGLIESRVEKIPRGRPRKYYMIKKGLRLEILLTPTLFGSEMYEAKGVRKSPEYEQAKELIKSQEPINVKMRELAEFLHELNERIREIIEEKRELEEARILIETYIENTMRRLAEENRQIIEEIFRDIEKILPPGYARSLKEKFL

General information: TITO was launched using: RESULT: Template: 2P4W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 284 -49512 -174.34 -458.44 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -174.34 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_2P4W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P4W-query.scw PDB file : Tito_Scwrl_2P4W.pdb: