Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFFRNQLANVVEWEEFRDDMIFYKWNNREIKKGSRLIIRPGQDAVFLNNGRVEGVFQDDGDYDIESEIIPFLSTLKGFKFGFNSGMRAEVLFVNTKEFTVRWGTKQAINIPAAGMPGGMPIRANGTFNFKVQDYISLIDKIAGVKDQYFVEDIKTRIISILDQLLMKWITREGKDMFNLQANAFDIAKGIQEDLDMQLISDGMTVTGFQIMSFNYPQEVQDMITKNASYGMVGDVNRYQQISMTDGMASGKMSGSGAASDMAGMMMGMNMANQMMNQMNQNQQAQSSGPQSTGSGSKPNFCPNCGTKTGEANFCPNCGQKLV 2RPB Chain:A ((86-174)) -----------------------------------------------------------------------------------------------------------------------VVVTVDAVVYYQVIDPVKAVYNV-S--------DFLMAIVKLAQTNLRAIIGEM--ELDETLSGRDIINARLREELDKITDRWGVKITRVEIQRIDPPKD--------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2RPB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 297 -52569 -177.00 -590.66 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -177.00 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.351

(partial model without unconserved sides chains): PDB file : Tito_2RPB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RPB-query.scw PDB file : Tito_Scwrl_2RPB.pdb: