Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTWRKSYERWKQTEHLDLELKERLIELEGDEQALEDCFYKDLEFGTGGMRGEIGAGTNRMNIYTVRKASAGFAAYISKQGEEAKKRGVVIAYDSRHKSPEFAMEAAKTLATQGIQTY---VFDELRPTPELSFAVRQLNA-----YGGIVVTASHNPPEYNGYKVYGDDGGQLPPKEADIVIEQVNAIE--NELTITVDEENKLKEKGLIKIIGEDIDKVYTEKLTSISVHPELSEEVDVKVVFTPLHGTANKPVRRGLEALGYKNVTVVKEQELPDSNFSTVTSPNPEEHAAFEYAIKLGEEQNADILIATDPDADRLGIAVKNDQGKYTVLTGNQTGALLLHYLLSEKKKQGILPDNGVVLKTIVTSEIGRAVASSFGLDTIDTLTGFKFIGEKIKEYEASGQYTFQFGYEESYGYLIG-----DFARDKDAIQAALLAVEVCAFYKKQGMSLYEALINLFNEYGFYREGLKSLTLKGKQGAEQIEAILASFRQNP-PQKMAGKQVVTAEDYAVSKRTLLTESKEEAIDLPKSNVLKYFLEDGSWFCLRPSGTEPKVKFYFAVKGSSLEDSEKRLAVLSEDVMKTVDEIVESTAK 2Z0F Chain:A ((11-522)) ----------------------------YEATPDPQNPLEGVRFGTSGHRGSSLK--ATFTEAHVLAIAQAIAELRPSFG---ATGPLFLAKDTHALSEPAWATALSVFAAHGIEVRVEADG-DYTPTPLVSLAILEHNAHHEAKADGVLLTP--NPPEDGGFKYNPPTGGPANARITRAIEERANALLQEGLKGVKRL-PLR-EALARAKP--FDYAGLYVEKVAEAVDLEAI-RASGLRIGVDPLGGASLRVWERLAESHGLP---LEVV--------------------------LLALKDRFDLAIGNDPDADRHGIVTPR--G--LM-NPNHYLAAALHHLYTTRSW--P---GAKVGKTAVTSALLDRVAQALGREVYETPVGFKHFVAGLLE------GWLGFAGEESAGASFLRFDGRPFSTDKDGILMGLLAAELMAK---RGQAPDALYEALAEKLGRPYYARKDLPVSPEA----KARLARLSAKEVHPSTLAGEPVLQVLDRATG-------------NGEPLGGIKVVAAN-AWFAVRPSGTEDVAKVYAESFLGEA-----HLERVLEEATALLHKA------

General information: TITO was launched using: RESULT: Template: 2Z0F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2959 -170227 -57.53 -367.66 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -57.53 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.443

(partial model without unconserved sides chains): PDB file : Tito_2Z0F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z0F-query.scw PDB file : Tito_Scwrl_2Z0F.pdb: