Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVNGIHHVSALTADAQKNLDFYKKVLGLKLVKKSVNQDEPTMYHLFYGDEVANPGTELTFFEIPRIAPFHAGTNSISSIGLRVPGTEALHYWKERFEEQQVTHSGISKRAGRDILAFQDHEGQRLVLTADEEGKG-YGLPVKQSGIPEEFSFRGLGPVELTVPYAEPTLHVLTNILGFTEISREPVEGQGTAVILESGEGGAATEIHLIER-NDLPRERQGKGSVHHVAFRVRDEEELAGWHRIISREGFSNSGIVERYYFKALYFREPNGILFELSTDGPGFMVDENLDELGQTIALPPYLEHRRAEIEAKLKPIQ 1ZSW Chain:A ((26-336)) YEIKGHHHISMVTKNANENNHFYKNVLGLRRVKMTVNQDDPSMYHLFYGDKTGSPGTELSFFEIPLVGRTYRGTNAITRIGLLVPSEDSLHYWKERFEKFDVKHSEMTTYANRPALQFEDAEGLRLVLLVSNGEKVEHWETWEKSEVPAKHQIQGMGSVELTVRRLDKMASTLTEIFGYTEVSRND-----QEAIFQSIKG-EAFGEIVVKYLDGPTE-KPGRGSIHHLAIRVKNDAELAYWEEQVKQRGFHSSGIIDRFYFKSLYFRESNGILFEIATDGPGFTVDGDVEHLGEKLDLPPFLEDQRAEIEANLAPIE

General information: TITO was launched using: RESULT: Template: 1ZSW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1776 -120836 -68.04 -391.05 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -68.04 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.515

(partial model without unconserved sides chains): PDB file : Tito_1ZSW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZSW-query.scw PDB file : Tito_Scwrl_1ZSW.pdb: