Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MR-NKRRNRQIVVAVNGGKAVKAIFLFIVSLIVIF----VLSGVLTSLRPELRPSSDSFYGIAEELPGDVFAH---------LLQMENHYFASDLSQTDSSFHLSRLSL---KLATSINLEDPRSFLGRELPGFAQFDTEILLAGQGTDYTNMPAESPPPSKVMEEEREANLAEIEKQQTQSDNAQKD-PPKQTTGDKKVVFI------YHTHNTE---SYLPLLKGETDPD------------MARHSKANVTLVGDMFGQALESQGIGAT---------VNKTDIQSKLNKKGLNY-ARSYDESRPVVKDALASNKN--------LQY------------IIDIHRDSRR-----------KKDTTATIKGK-SYARVAFVVGKKSKNFEENY-------KIASELHKLMEKKYPGLSTGVFSKGSPG----DNGVYNQDLTDRALLLEFGGVDNNLEELQRAANAAADV------FSEMYWDAEKVNAASGETKKQ 3J2P Chain:A ((1-495)) MRCIGISNRDFVEGVSGGSWVDIVLEHGSCVTTMAKNKPTLDFELIETEAK-QPATLRKYCIEAKLTNTTTDSRCPTQGEPSLNEEQDKRFVCKHSMVDRGWGNGCGLFGKGGIVTCAMFTCKKNMKGKVVQP-ENLEYTIVITPHSGEEHAVGNDTGKHGK--EIKITPQSSITEAELTGYGTVTMECSPRTGLDFNEMVLLQMENKAWLVHRQWFLDLPLPWLPGADTQGSNWIQKETLVTFKNPHAKKQDVVVLGSQEGAMHTALTGATEIQMSSGNLLFTGHLKCRLRMDKLQLKGMSYSMCTGKFKVVKEIAETQHGTIVIRVQYEGDGSPCKIPFEIMDLEKRHVLGRLITVNPIVTEKDSPVNIEAEPPFGDSYIIIGVEPGQLKLNWFKKGSSIGQMIETTMRGAKRMAILGDTAWDFGSLGGVFTSIGKALHQVFGAIYGAAFSGVSWIMKILIGVIITWIGMNSRSTSLSVSLVLVGVVTLYLGVMVQA

General information: TITO was launched using: RESULT: Template: 3J2P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2142 81099 37.86 204.28 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 37.86 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.164

(partial model without unconserved sides chains): PDB file : Tito_3J2P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3J2P-query.scw PDB file : Tito_Scwrl_3J2P.pdb: