Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIAIYVRVSTEEQAIKGSSIDSQIEACIKKAGTKDVLKYADEGFSGELLERPALNRLREDASKGLISQVICYDPDRLSRKLMNQLIIDDELRKRNIPLIFVNGEYANSPEGQLFFAMRGAISEFEKAKIKERTSSGRLQKMKKGMIIKDSKLYGYKFVKEKRTLEILEEEAKIIRMIFNYFTDHKSPFFGRVNGIALHLTQMGVKTKKGAKVWHRQVVRQILMNSSYKGEHR-QYKYDTEGSYVSKQAGNKSIIKIRPEEEQITVTIPAIVPAEQWDYAQELLGQSKRKHLSISPHNYLLSGLVRCGKCGNTMTGKKRKSH----GKDYYVYTCRKNYSGAKDRGCG-KEMSENKLNRHVWGEIFKFITNPQKYVSFKEAEQSNHLSDELELIEKEIEKTKKGRKRLLTLISLSDDDDLDIDEIKAQIIELQKKQNQLTEKCNEIQSKMKVLDDTSSSENALKRAIDYFQSIGADNLTLEDKKTIVNFIVKEVTIVDSDTIYIETY------- 4KIS Chain:A ((2-328)) ----------------------------------------------------------------------------------------------------------------------------------DRMVMGKIKRIEAGLPLTTAKGRTFGYDVIDTKLYINEEEAKQLRLIYDIFEEEQS-----ITFLQKRLKK------LGFKVRTYNRYNNWLTNDLYCGYVSYKDKVHVKGIH------------------------EPIISEEQFYRVQEIFSRMGKNPNMNKESASLLNNLVVCSKCGLGFVHRRKDTVSRGKKYHYRYYSCKTYKHTHELEKCGNKIWRADKLEELIIDRVNNYSFAS------------RNIDKEDESLNEKLKIEHAKKKRLFDLYI---NGSYEVSELDSMMNDIDAQINYYEAQIEANEELKKNKTVD------------------FNSLEFREKQLYLKSLINKIYIDGEQVTIEWLLEHHHHHH

General information: TITO was launched using: RESULT: Template: 4KIS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 952 3312 3.48 10.97 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 3.48 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.410

(partial model without unconserved sides chains): PDB file : Tito_4KIS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4KIS-query.scw PDB file : Tito_Scwrl_4KIS.pdb: