Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRLDKLLANSGYGSRKEVKAVVKAGAVMIDGKPAKDVKEHVDPDTQEVTVYGEPVDYREFIYLMMNKPQGVLSATEDS-RQQTVVDLLTPEEMRFEPFPAGRLDKDTEGFLLLTNDGQLAHRLLSPKKHVPKTYEVHLKSQISREDISDLETGVYIEG--------GYKTKPAKAEIK-----T-NDSGNTVIYLTITEGKYHQVKQMAKAVGNEVVYLKRLSMGRVSLDPALAPGEYRELTEEELHLLNEPQA 2OLW Chain:A ((37-216)) ---------------------------------------------------------QPTRVILFNKPYDVLPQFTDEAGRKTLKEFIP----VQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPGKRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIR-RKSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLD-NLANGEWREVT------------

General information: TITO was launched using: RESULT: Template: 2OLW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 736 13808 18.76 84.19 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 18.76 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.509

(partial model without unconserved sides chains): PDB file : Tito_2OLW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OLW-query.scw PDB file : Tito_Scwrl_2OLW.pdb: