Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------MRVFVARQPIFNRKEQVVAYELLYRESEENVYSAKDGDQATT--DLVINSFLNIGIEKLTEGKRCFV-NFTESLMFSNLPTSFNPKQLVIEILEDIP-------ITPALISRCKELKKMGYMLALDDFYAINP--QDEDLLEKLMSYID---------ILKIDFL--------KTTRMERRTILQTYGCRGLIFLA--EKVETRKEYKQAVQDGFQLFQGYFFSEPRIISGHDLSTHFYSYYELL--NELSKEQPNIKRVTEYIERDLSLSYQILKFLNSSHSRLSQKIESIQQA--------IMLLGFNEIKRWIY-----ILSFKDLSRKGHS----SKHEIIKISLIRAKLCELL---------ARKTSRPQPASYMLIGMFSLIDTL------LHREIEEIVQELP--LKDEVGQALLGHQNDYYQMLE--LVKLIE--SNNWDTCSELGNQLDKE----EAYECYLEALEWCHNLMDAK-------- 5DSE Chain:A ((140-843)) VIAEAYATKGLCLEDVITCYEKAGDIALLYLQEIERVLGFFLETGLQRAHVLYFKNGNLTRGVGRFRELLRAVETRTTQNLRMTIARQLAEILLRGMCEQSYWNPLEDPFCPQENTEEALLLLLISESMANRSVVYDLLTIALGRRGQYEMLSECLERAMKFAFEEFHLWYQFALSLMAAGKSARAVKVLKECIRLKPDDATIPLLAAKLCMGSLHWLEEAEKFAKTVVTSEFKAKGYLALGLTYSLQATDASLRGMQEVLQRKALLAFQRAHSLSPTDHQAAFYLALQLAISRQIPEALGYVRQALQLQGDD-ANSLHLLALLLSAQKHYHDALNIIDMALSEYPENFILLFSKVKLQSLCRGPDEALLTCKHMLQIWKSCYLHPWMTLAQIWLHAAEVYIGIGKPAEATACTQEAANLFPMSHNVLYMRGQIAELRGSMDEARRWYEEALAISPTHVKSMQRLALILHQLGRYSLAEKILRDAVQVNSTAHEVWNGLGEVLQAQGNDAAATECFLTALELEASSPAVPFTIIPRVL

General information: TITO was launched using: RESULT: Template: 5DSE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1836 -41858 -22.80 -102.59 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -22.80 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.248

(partial model without unconserved sides chains): PDB file : Tito_5DSE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DSE-query.scw PDB file : Tito_Scwrl_5DSE.pdb: