Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MKIKTISGAERLSLK-RSIDAGTEEQRKTVRSIIEDVKANGDQAVRSYTAKFDCIEIDSPLVTKEEFEEAYTSLDSRLLQV-IRQAIENIREYHERQLQSSWFYHRKDGTMLGQKVTALDSAGVYVPGGTAAYPSSVL-MNVIPALVAGVERIVLVTPPGKDGLLSPGVLVAAAELGIKDIYKMGGAQAIAALAYGTETIEPVDKITGPGNIYVALAKREVFGDVDIDMI---AGPSEIVVLADETAIPSEIAADLLSQAEHDKLSSCVFVTDSMAL-AETVSAEVNKQLETLPRREIAEASVRDYGCIYVAETMVEAIETVNTLAPEHLEIITQSPEALLGSIKHAGAIFLGRYSPEPVGDYFAGPNHVLPTNGTARFSSPLNVTDFQKK-SSIISYSQSAFEEHAESIAAFARLEGLEAHARSIEARERRISK-- 1K75 Chain:A ((4-434)) NTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVKARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMA-VAVKNIETFHTAQKLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLML-ATPASIAGCKKVVLCSPPP----IADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAKRQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPA-ADMARRVAEAVERQLAELPRAETARQALNASRLIVT-KDLAQCVEISNQYGPEHLIIQTRNARELVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMT-VQELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA

General information: TITO was launched using: RESULT: Template: 1K75.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2178 5458 2.51 13.06 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : 2.51 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.514

(partial model without unconserved sides chains): PDB file : Tito_1K75.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1K75-query.scw PDB file : Tito_Scwrl_1K75.pdb: