Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYSTIFNIGQINKYSKLAIFMSILFLCGCSSQTHSSQKETTIPVTLHVED-AKGLPVEGVQVTIVKAPSSDEEPSTEIGEILGKTDKNGDIK-W----DTGRKGDYSVALTKGETSVTHHISLTEDKKDHAIPLVFKE 4Q14 Chain:A ((22-93)) ----------------------------------------MGKLSTHVLDTAHGTPAAAMRVELYRIAAS----GTPELLKRVVTNLDGRTDAPLLSGDEMRTGIYELQFHVAEYF----------------------

General information: TITO was launched using: RESULT: Template: 4Q14.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 214 -12795 -59.79 -193.86 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -59.79 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.496

(partial model without unconserved sides chains): PDB file : Tito_4Q14.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q14-query.scw PDB file : Tito_Scwrl_4Q14.pdb: