Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIISYKCPNCGSDMAFDSETGSLSCSSCGRQDNIESLPKENIAARFSDDEAKEYQCKNCGAVLITEAETTATTCSFCGGAAILADRLSGHLAPAKVIPFTISKQEAEQAFRKWCKKGLLTPRGFMSADRIKSITGMYIPFWMFDLNSEVQVRANCTRVHQYEEGDYICTETEHFEAFRDINLDYLKIPVDASEKMKDELMDKLEPYSYEELKDFQTAYLAGYIAEKYNYTD-----EELFPRAKEKISSYIDSYIHSTFSGYTSVNVRDKHIHTKNVNSFYVLLPVWMVSYDYERAEHIFAMNGQTGKVVG-KPPISRGKVAAWFSGIAGGTFLALKLVSLMMGGGF------------ 4O6M Chain:A ((29-370)) MRLAYVK----NHEIYGEKLLGLT----LRERIEKTLQRAGFDVRFFDELSLE---EAEDYLIILEPVLILERDLLLEGRKILVSD-----------GFTVGYFFGGDFRTVFDGNLQSSIEKYLSLNNLESYE-----IWAIKLSNDNLKTAEKLLLSSLIDG-WIAREINRKVSLRISRLLA-DTSVTPNQITVFSFFLSLVGSALFLLNSYLTTLLAGVIIQLHSIIDGCDGEIARLKFMESKYGAWLDGVLDRYSDFIIVFSITYVLSASNPVYWIIGFLAAFASLMIAYTGDKFVAAYMRTYSPEGFAIPITRDFRLLIIFACSVVNLPSLALVIIALLGNFEALRRIVALRSY

General information: TITO was launched using: RESULT: Template: 4O6M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1424 -115798 -81.32 -371.15 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -81.32 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.121

(partial model without unconserved sides chains): PDB file : Tito_4O6M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4O6M-query.scw PDB file : Tito_Scwrl_4O6M.pdb: