Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEALTEQKSQKWAISLFTIGVFMAALDNGIISAALTTINESFSVSPSWGSWGITLYTLGLSVSVPIVGKLSDRYGRKKLFLIEVCLFGLGSLLVAL----SQSFPLFLISRLIQALGGGGIFIIGSSHILATLPK--EKQGKALGLLGAMNGMAAVLGPNIGSFLLDWTGSWHWLFLINLPIAVLLVVFGACFIAETKAPEAKRLDAAGIFLLSLSILAVMYGMTNLDGANLLHSLGNPEVYGCIIFGILCFAALISYEKRVEMRGGDPILAYSLLRNHMFQRTLIIGLLSGGLLAAVIFIPSYVEQYLGVPAAKAGYWMTPLALASGIGAWLGGALTDKKGPVKTVILSGIISCAGFALFPLWVTEKWEFVIASVAAGIGFGFLLGAPLNVLVSEAAKTNKGTALGTLSLVRQIGLTLAPTLYAGFITAGFDQIGDEINSRLSDSGYSEKAMQMIPEIDSSEVSSLQEQIERIPVPEVKTAISDAIHASVASGYDHLYAAAAVVSLLVIAAISIPAFRRQKR 1PV6 Chain:A ((1-417)) -----MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDTGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALGASIVGIMFTI--NNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVG------------------------------------------------ANHSAFSLKLALELFRQPKLW-FLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPLIINRIGGKNALLLAGTIMSVRIIGSSF-ATSALEVVILKTLHMFEVPFLLVGCFKYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESI--------------GFQGAYLVLGLVALGFTLISVFTLSGPGPLSLLRRQVNEVA------------------------------------

General information: TITO was launched using: RESULT: Template: 1PV6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1919 -266249 -138.74 -647.81 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -138.74 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.207

(partial model without unconserved sides chains): PDB file : Tito_1PV6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PV6-query.scw PDB file : Tito_Scwrl_1PV6.pdb: