Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGIAGTFIFMIVIGAAIGAVTNHLAIQMLFRPYKAYYLFGKRVPFTPGLIPRRRDELAKQMGLMVVNHLLTPEGIKKRLVSDAAKTQALRVGEQLIQKLSLSEVTVKEALEKAGMKRPEKAADAWISSWTDDKLHELFRQYGDQSLKELVPIEVQEKLEEKIPMISGYILSRSVRYFESDEGKIRLGNMIDDFLKERGMLGSMVQLFLGNSSLADRVLPELLKFLRNEETNKLLSDLLKNEWGKLREYTFNEADEKWNAKALIFSLKRRVLQAFS---TAPFFNNTIGTLTVRYESELTQQMLPALLDKLLEGISSNLESVLKRLRLEEIVKEQVDQFPVERLEEMVLSISKKEFKMITYLGGLLGGIIGAIQALFVILF 4DO7 Chain:A ((3-289)) --------------------ALRIDSHQHFWRYR-----AADYPW----IGAGMGVLARDYLPDALHPLMHAQALG---ASIAVQARAGRDETAFLLELACDEARIAAVVGWEDLRAPQ--LAERVAEWRGTKLRGFRHQLQDEA-------DVRAFVDD-------ADFARGVAWLQAN-------DYVYDVL------------------VFERQLPDVQAFCARHDAHWLVLDHA----GKPALAEFDTALARWRAALRELAALPHVVCKLSGLVTEADWRRGLRASDLRHIEQCLDAALDAFGPQRLMFGSDWPVCLLAA-SYDEVASL-VERWAESRL-------SAAERSAL------WGGTAARCYALP----

General information: TITO was launched using: RESULT: Template: 4DO7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1515 -130972 -86.45 -466.09 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -86.45 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.219

(partial model without unconserved sides chains): PDB file : Tito_4DO7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DO7-query.scw PDB file : Tito_Scwrl_4DO7.pdb: