Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVFLIGANGQIGQRLVSLFQDNP-DHSIRAMVRKEEQKASLEAAGAEAVLANLEGSPEEIAAAAKGCDAIIFTAGSGGSTGYDKTLLVDLDGAAKAIEAAAIAGIKRFIMVSALQAHNRENWNEALKPYYVAKHYADKILEASGLTYTIIRPGGLRNEPGTG---------TVSA-AKDLERGFISRDDVAKTVIASLDEKNTENRAFDLTEGDT-PIAEALKKL 2JL1 Chain:A ((1-212)) FSIAVTGATGQLGGLVIQHLLKKVPASQIIAIVRNVEKASTLADQGVEVRHGDYN-QPESLQKAFAGVSKLLFISGPHYD------NTLLIVQHANVVKAARDAGVKHIAYTGYAFAEE------SIIPLAHVHLATEYAIRTTNIPYTFLRNALYTDFFVNEGLRASTESGAIVTNAGSGIVNSVTRNELALAAATVLTEEGHENKTYNLVSNQPWTFDELAQI-

General information: TITO was launched using: RESULT: Template: 2JL1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1013 -131533 -129.85 -657.67 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -129.85 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_2JL1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JL1-query.scw PDB file : Tito_Scwrl_2JL1.pdb: