Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------MLQLNGKDVKWKKDTGTIQDLLASYQLENKIVIVERNKEIIGKERYHEVELCDRDVIEIVHFVGGG 1RYJ Chain:A ((4-73)) MVIGMKFTVITDDGKKILESGAPRRIKDVLGELEIPIETVVVKKNGQIVIDEE----EIFDGDIIEVIRVIYGG

General information: TITO was launched using: RESULT: Template: 1RYJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 193 -24226 -125.52 -390.73 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -125.52 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.395

(partial model without unconserved sides chains): PDB file : Tito_1RYJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RYJ-query.scw PDB file : Tito_Scwrl_1RYJ.pdb: