Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLYSKKDIVQQARNLAKMISETEEVDFFKRAEAQINENDKVST-IVNQIKALQKQAVNLKHYEKHEALKQV-EAKID-A-LQEELEEIP-VIQEFRDSQMEVNDLLQLVAHTISNQVTNEIITSTGGDLLKGETGSKVKHSNNSCSL 2OEE Chain:A ((3-112)) ------VNFYDVAYDLENALRGSEEFTRLKNLYDEVNADESAKRMFENFRDVQLQAQKTVALVQQHEKISQLMEAEQRMSMLIGELNKIIMKPLEELY--------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2OEE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 140 -12987 -92.76 -149.27 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -92.76 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.591

(partial model without unconserved sides chains): PDB file : Tito_2OEE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OEE-query.scw PDB file : Tito_Scwrl_2OEE.pdb: