Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLIILEGPDCCFKSTVAAKLSKELKYP-----IIKGSSF--ELAKSG-------NE----KL-FE--H--------FNKLADEDNVIIDRFVYSNLVYAKKFKDYSILTERQLRFIE--DKIKAKAK-VVYLHADPSVIKKRLRVRGDEYIEGKDIDSILELYREVMSNAGLHTYSWDTGQWSSDEIAKDIIFLVE 4S35 Chain:A ((1-190)) -MLIAFEGIDGSGKTTQAKKLYEYLKQKGYFVSLYREPGGTKVGEVLREILLTEELDERTELLLFEASRSKLIEEKIIPDLKRDKVVILDRFVLSTIAYQGY--GKGL-DVEFIKNLNEFATRGVKPDITLLLDIPVDIALRRLKEKNRFENK-EFLEKVRKGFLELAKE-EENVVVIDASG-EEEEVFKEILRALS

General information: TITO was launched using: RESULT: Template: 4S35.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 725 41961 57.88 265.57 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 57.88 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_4S35.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4S35-query.scw PDB file : Tito_Scwrl_4S35.pdb: