Modeling by threading (Tito software)
Unconserved sides chains calculation (Scwrl software)
Evaluation (QMean software)



Input alignment information:
Query sequence---------------------------MGQKFHKLNSKYGIIDYPILLKDLESIIQEFPKSERKFYEYAIKALKKEVGKKEKILHMTSADPKLTKFGFMVITEKKLLFVTMKGGFFGGADTEVVEFKSIKEVDFDIAPNPLGMATMQLGILHLKIKGKLGMSSKRTIRNIDEHSLDKIVAILREQTK--------------------------------------------------------------------------------------
2MHK Chain:A ((1-250))MGSSHHHHHHSSGLVPRGSHMGTHTPDQSTAYMQGTAQA---DSAFYLQQMQ---QSSDDTRINWQLLAIRALVKE-GKTGQAVELFNQLPQ--ELNDAQRREKTLLAVEIK--------LAQKDFAGAQNLLAKITPADLE-QNQQARYWQAKIDASQGRPSIDLLRALIAQE-----PLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDPDMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPAST


General information:
TITO was launched using:
RESULT:

Template: 2MHK.pdb
Alignment : align.pir
Tito was launched with SMD and SCWRL
Tito text output
Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 555 42585 76.73 310.84
target 2D structure prediction score : 0.39
Monomeric hydrophicity matching model chain A : 0.62

3D Compatibility (PKB) : 76.73
2D Compatibility (Sec. Struct. Predict.) : 0.39
1D Compatibility (Hydrophobicity) : 0.62
QMean score : 0.296

(partial model without unconserved sides chains):
PDB file : Tito_2MHK.pdb:





(Unconserved sides chains are recalculated) :
Sequence: align-2MHK-query.scw
PDB file : Tito_Scwrl_2MHK.pdb: