Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------MIRGILIAVLGIAIVGTGYWGYKEHQEKDAVLLHAENNYQRAFHELTYQ----------VDQLHDKIGTTLAMNSQKSLSPALIDVWRITSEAHNSVSQLPLTLMPFNKTEE---LLSKIGDFSYKTSVRDLDQKPLDKN-EYTSLNKLYQQS-----EDIQNELRHVQHLVMSKNLRWMDVEMALASDEKQSDNTI-INSFKTVEKNVGAFSTGTDLGPSFTSTKKEEKGFSHL---KGKQISEQEAKQIAERFAPDDNYSIKVVKSGKKTNRDVYSISMKDPDHKAVIYM--DITKKGGHPVYLIQNREVKDQKISLNDGSNRALAFLKK-NGFETDDLEIDESAQYDKIGVFSYVPVENKVRMYPEAIRMKVALDDGEVVGFSARDFLTSHRK------RTIPKPAITEAEAKSKLNKNVQVRE-TRLALITNELGQEVLCY-----EMLGTIENDTFRMYINAKDGSEEKVEKLKNAEPIYKDL------------------------------------------------------------------ 5DSE Chain:A ((140-843)) VIAEAYATKGLCLEDVITCYEKAGDIALLYLQEIERVLGFFLETGLQRAHVLYFKNGNLTRGVGRFRELLRAVETRTTQNLRMTIARQLAEILLRGMCEQSYWNPLEDPFCPQENTEEALLLLLISESMANRSVVYDLLTIALGRRGQYEMLSECLERAMKFAFEEFHLWYQFALSLMAAGKSARAVKVLKECIRLKPDDATIPLLAAKLCMGSLHWLEEAEKFAKTVVTSEFKAKGYLALGLTYSLQATDASLRGMQEVLQRKALLAFQRAHSLSPT------------DHQAAFYLALQLAISRQIPEALGYVRQALQLQGD-DANSLHLLALLLSAQKHYHDALNIIDMALSE---------YPENFILLFSKVKLQSLCRGPDEALLTCKHMLQIWKSCYLHPWMTLAQIWLHAAEVYIGIGKPAEATACTQEAANLFPMSHNVLYMRGQIAELRGS-MDEARRWYEEALAISPTHVKSMQRLALILHQLGRYSLAEKILRDAVQVNSTAHEVWNGLGEVLQAQGNDAAATECFLTALELEASSPAVPFTIIPRVL

General information: TITO was launched using: RESULT: Template: 5DSE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1952 71311 36.53 167.00 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 36.53 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.181

(partial model without unconserved sides chains): PDB file : Tito_5DSE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DSE-query.scw PDB file : Tito_Scwrl_5DSE.pdb: