Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDIRTITSSDYEMVTSVLNEWWGGRQ----L----KEKLPRLFFEHFQDTSFITSEHNSMTGFLIGFQSQS---DPETAYIHFSGVHPDFRKMQIGKQLYDVFIETVKQRGCTRVKCVTSPVNKVSIAYHTKLGFDIEKGTKTVNGISVFANYDGPGQDRVLFVKNI 2FE7 Chain:A ((3-141)) LEIRPAVPADAEQILAFIIELADYERARHEVVTDVEGIRRSLFAEGSPTRALMCLSEGRPIGYAVFFYSYSTWLGRNGIYLEDLYVTPEYR--GAGRRLLRELAREAVANDCGRLEWSVLDWNQPAIDFYRSIGALPQD----------------------------

General information: TITO was launched using: RESULT: Template: 2FE7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 485 1110 2.29 8.81 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 2.29 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.558

(partial model without unconserved sides chains): PDB file : Tito_2FE7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FE7-query.scw PDB file : Tito_Scwrl_2FE7.pdb: