Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MARKLFTPITIKDMTLKNRIVMSPMCMYSSHEKDGKLTPFHMAHYISRAIGQVGLIIVEASAVNPQGRITDQDLGIWSDEHIEGFAKLTEQVKEQGSKIGIQLAHAGRKAE----LEGD------------IFAPSAIAFDEQ-SATPVEMSAEKVKETVQEFKQAAARAKEAGFDVIEIHAAHGYLIHEFLSPLSNHRTDEYGGSPENRYRFLREIIDEVKQVW--DGPLFVRVSASDYTDKG-LDIADHIGFAKWMKEQGVDLIDCSSG-ALVHADINVFPGYQVSFAEKIREQADMATGAVGMITDGSMAEEILQNGRADLIFIGRELLRDPFFARTAAKQLNTE-----IPAPVQYERGW 4UTL Chain:A ((6-359)) ----LFEPYTLKDVTLRNRIAIPPMCQYMA--EDGMINDWHHVHLAGLARGGAGLLVVEATAVAPEGRITPGCAGIWSDAHAQAFVPVVQAIKAAGSVPGIQIAHAGRKASANRPWEGDDHIAADDTRGWETIAPSAIAFGAHLPKVPREMTLDDIARVKQDFVDAARRARDAGFEWIELHFAHGFLGQSFFSEHSNKRTDAYGGSFDNRSRFLLETLAAVREVWPENLPLTARFGVLEYDGRDEQTLEESIELARRFKAGGLDLLSVSVGFTIPDTNIPWGPAFMGPIAERVRREAKLPVTSAWGFGTPQLAEAALQANQLDLVSVGRAHLADPHWAYFAAKELGVEKASWTLPAPYAH----

General information: TITO was launched using: RESULT: Template: 4UTL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1983 -10593 -5.34 -32.29 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -5.34 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.711

(partial model without unconserved sides chains): PDB file : Tito_4UTL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UTL-query.scw PDB file : Tito_Scwrl_4UTL.pdb: