Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLYGVIGNPIGHSMSPDIHNASLKDLGLDGHYHAFKVEENDLEDAVKGIRALGVQGINVTVPHKVSIMDYLDHIDESAKVLGAVNTVRREGD-KLVGYNTDGEGFVKSLM--KVLDKPISELSFLMIGAGGAARAIFTTIVRNTPKKFDICNRTLEKAKRLTEATPSFHNKEVLSIKEAEERLEQ-YDVIIHTTSVGMYPNVDDVPLSLQRAASSAVVCDIVYNPIQTALLKEASQKGLKTLDGVGMFVEQAALSFQLWTGQEPNIEKMRSIVIGKLGGTEC 3SEF Chain:C ((32-290)) ----YAVFGNPINHSKSPFIHTLFARQTQQSMIYTAQCVPVDGFTEAAKHFFAQGGRGCNVTVPFKEEAYRFADRLTERARLAGAVNTLKK-DDGEILGDNTDGEGLVQDLLAQQVLLKGA---TILLIGAGGAARGVLKPLLDQQPASITVTNRTFAKAEQLAELVAAYGEVKA----QAFEQLKQSYDVIINSTS-----AIDPVIFS-----SRSVCYDMMYG-----------------IDGLGMLVGQAAESFMLWRGLRPGTKQI-------------

General information: TITO was launched using: RESULT: Template: 3SEF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1254 -47183 -37.63 -206.94 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain C : 0.76 3D Compatibility (PKB) : -37.63 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.541

(partial model without unconserved sides chains): PDB file : Tito_3SEF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SEF-query.scw PDB file : Tito_Scwrl_3SEF.pdb: