Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIGFFDSGIGGMTVLYEAIKVLPYEDYIFYADTLNVPYGEKSKGKVKEYIFNAAEFLASQNIKALVIACNTATSIAIEDLRRNFDFPIIG-IEPAVKPAINKCTEERKRVLVVATNLTLKEEKFHNLVKEIDHHDLVDCLALPGLVEFAENFDFSEDKIIKYLKNELSSFDLKQYGT--------IVLGCTHFPFFKNSFEKLFGIKVDMISGSVGTAKQLKKVLADRNQLGKGSGSITFFNSGHKIVDQEVISKYKRLFEILDETQRSHVGH 2JFY Chain:B ((1-195)) MKIGVFDSGVGGFSVLKSLLKARLFDEIIYYGDSARVPYGTKDPTTIKQFGLEALDFFKPHEIELLIVACNTASALALEEMQKYSKIPIVGVIEPSILAIKRQVEDKNAPILVLGTKATIQSNAYDNALKQQGYLN-ISHLATSLFVPLIEE---------SILEGELLETCMHYYFTPLEILPEVIILGCTHFPLIAQKIEGYF---------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2JFY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 954 -80839 -84.74 -434.62 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : -84.74 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.554

(partial model without unconserved sides chains): PDB file : Tito_2JFY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JFY-query.scw PDB file : Tito_Scwrl_2JFY.pdb: