Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------MKIYGVYMDRPL---SAGEEDRMMAAVSAEKREKCRRFYHKEDAHRTLIGDMLIRTAAAKAYGLDPAGISFGVQEYGKPYI-----PALPDMHFNISHSGRWIVCAV-DSKPIGIDIEKMKP-------GTIDIAKRFFSPTEYSDLQA-KHPDQQTDYFYHLWSMKESFIKQAGKGLSLPLDSFSVRLKDDGH---------VSIELPDGHEPCFIRTYDADEEYKLAVCAAHPDFCDGIEMKTYEELL------------------------------ 2BYD Chain:A ((18-318)) LYFQGHMEGVRWAFSCGTWLPSRAEWLLAVRSIQPEEKERIGQFVFARDAKAAMAGRLMIRKLVAEKLNIPWNHIRLQRTAKGKPVLAKDSSNPYPNFNFNISHQGDYAVLAAEPELQVGIDIMKTSFPGRGSIPEFFHIMKRKFTNKEWETIRSFKDEWTQLDMFYRNWALKESFIKAIGVGLGFELQRLEFDLSPLNLDIGQVYKETRLFLDGEEEKEWAFEESKIDEHHFVAVALRKQR---QFTILNFNDLMSSAVPMTPEDPSFWDCFCFTEEIPIRNGTK

General information: TITO was launched using: RESULT: Template: 2BYD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 851 31414 36.91 142.14 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 36.91 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.512

(partial model without unconserved sides chains): PDB file : Tito_2BYD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BYD-query.scw PDB file : Tito_Scwrl_2BYD.pdb: