Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEQPIGVIDSGVGGLTVAKEIMRQLPKENIIYVGDTKRCPYGPRPEEEVLQYTWELTNYLLENHHIKMLVIACNTATAIALDDIQRSVGIPVVGVIQPGARAAIKVTDNQH--IGVIGTENTIKSNAYEEALLALNPDLKVENLACPLLVPFVESGKFLDKTADEIVKTSLYPLKDTSIDSLILGCTHYPILKEAIQRYMGEHVN------IISSGDETAREVSTILSYKGLLNQSPIAPDHQFLTTGARDQFAKIADDWFGHEVGHVECISLQEPIKR 2JFZ Chain:B ((3-253)) -----IGVFDSGVGGFSVLKSLLKARLFDEIIYYGDSARVPYGTKDPTTIKQFGLEALDFF-KPHEIELLIVACNTASALALEEMQKYSKIPIVGVIEPSILAIKRQVEDKNAPILVLGTKATIQSNAYDNA-LKQQGYLNISHLATSLFVPLIEESILEGELLETCMHYYFTPLEILP-EVIILGCTHFPLIAQKIEGYFMGHFALPTPPLLIHSGDAIVEYLQQKYALK---------PKVEFHASGDVIWLERQAKEWL------------------

General information: TITO was launched using: RESULT: Template: 2JFZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1361 -92845 -68.22 -391.75 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -68.22 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.535

(partial model without unconserved sides chains): PDB file : Tito_2JFZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JFZ-query.scw PDB file : Tito_Scwrl_2JFZ.pdb: