Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENFTYYNPTKLIFGKGQLEQLRKEFKRYGKNVLLVYGGGSIKRNGLYDQVTGILKEEGAVVHELSGVEPNPRLATVEKGIGLCREHDIDFLLAVGGGSVIDCTKAIAAGVKYDG-DAWDIFSKKVTAEDALPFGTVLTLAATGSEMNPDSVITNWETNEKFVWGSNVTHPRFSILDPENTFTVPENQTVYGMVDMMSHVFEQYFHNVENTPLQDRMCFAVLQTVIETAPKLLEDLENYELRETILYAGTIALNGTLQMG-YFGDWASHTMEHAVSAVYDIPHAGGLAILFPNWMRYTLDTNVGRFKNLMLNMFDIDTEGKTDKEIALEGIDKLSAFWTSLGAPSRLADYNIGEEKLELIADIAAKEMEHGGFGNFQKLNKDDVLAILRASL 1O2D Chain:A ((15-369)) ----EFYMPTDVFFGEKILEKRGNIIDLLGKRALVVTGKSSSKKNGSLDDLKKLLDETEISYEIFDEVEENPSFDNVMKAVERYRNDSFDFVVGLGGGSPMDFAKAVAVLLKEKDLSVEDLYDRE-KVKHWLPVVEIPTTAGTGSEVTPYSILTDPE-GNKRGC--TLMFPVYAFLDPRYTYSMSDELTLSTGVDALSHAVEGYLSRKST-PPSDALAIEAMKIIHRNLPKAIE--GNREARKKMFVASCLAGMVIAQTGTT----LAHALGYPLTTEKGIKHGKATGMVLPFVMEVMKEEIPEKVDTVNHI-FGG----------------SLLKFLKELGLYEKV---AVSSEELEKWVEKGSRAKH--LKNTPGTFTPEKIRNIYREAL

General information: TITO was launched using: RESULT: Template: 1O2D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2177 -81815 -37.58 -231.77 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -37.58 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.494

(partial model without unconserved sides chains): PDB file : Tito_1O2D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1O2D-query.scw PDB file : Tito_Scwrl_1O2D.pdb: