Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEYGFKDDSLSL-HTDLYQINMAETYWRDGIHEKKAIFELFFRRLPFENGY--AVFAGLEKAI-EYLENFKFTDSDLSYLQDELGYH-------EDFIEYLRGL---SFTGSLYSMKEGELVFNNEPIMRVEAPLVEAQLIET---ALLNIVNYQTLIATKAARIKGVIGD-------------EVALEFGTRRAHEMDAAMWGARAALIGGFSATSNVRAGKRFNI--------PVSGTHA--HALVQAY--RDEYTAFKKYAETHKD--CVFLVDTYDT---LRSGMPNAIRVAKEFGDRINFIGIRLDSGDL-AYLSKKARKMLDEAGFT-----------DAKVIASSDLDEHT---IMNL-KAQGARID--VWGVGTKLITAYDQPALGAVYKLVAIEEDGKMVDTIKIS-SNPEKVTTPGRKK-VYRIINQSNHHSEGDYIALYDEQVNDQKRLRMFHPVHTFISKFVTNFYAKDLHELIFEKGILCYQNPEISDIQQYVQDNLSLLWEEYKRISKPEEYPVDLSEDCWSNKMQRIHEVKSRIEEELEEE 2H3B Chain:A ((11-486)) --------EFNILMATDSYKVTHYKQYP-------PNTSKVYSYFECREKKYEETVFYGLQYILNKYLKGKVVTKEKIQEAKEVYREHFQDDVFNERGWNYILEKYDGHLPIEVKAVPEGSVIPRGNVLFTVENTDPECYWLTNWIETILVQSWYPITVATNSREQKKILAKYLMETSGNLDGLEYKLHDFGYRGVSSQETAGI-GASAHLVNFKGTDTVAGIALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGEDLRHLIVSRSTEAPLIIRPDSGNPMDTVLKVLDILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKKWSIENVSFGSGGALLQKLTRDLLNCSFKCSYVVTNGLGVNVFKDPVADPNKRSKKGRLSMHRT--------PAGNFVTLEEGKGDLEEY-------------------GHDLLHTVFKNGKV-TKSYSFDEVRKNAQLN-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 2H3B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2120 57320 27.04 143.66 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 27.04 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.405

(partial model without unconserved sides chains): PDB file : Tito_2H3B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2H3B-query.scw PDB file : Tito_Scwrl_2H3B.pdb: