Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLNLQGKTALVTGSTSGIGKAIASSLAEEGAAVIINGRREE-KVNQTIDELKTQHAEAVLYPAAFDLGTEEGCNELF----QAYPEVDILVNNLGIFEPAEYFDIPDDEWFRFFEVNIMSGVRLTRRYLHNMIEKKEGRVIFIASEAAIMPSQEMAHYSATKTMQLSISRSLAELATGTNVTVNTVMPGSTLTEGVETMLNSLYPGENLTVQEAEAR-FMKENRPTSIIQRLIRPEEIAHFVTFLSSPLSSAINGAALRADGGLVRSVF 2ZTV Chain:A ((2-256)) ----LKGKVAVVTGSTSGIGLGIATALAAQGADIVLNGFGDAAEIEKVRAGLAAQHGVKVLYDGA-DLSKGEAVRGLVDNAVRQMGRIDILVNNAGIQHTALIEDFPTEKWDAILALNLSAVFHGTAAALPHMKKQGFGRIINIASAHGLVASANKSAYVAAKHGVVGFTKVTALETAGQGITANAICPGWVRTPLVEKQISALAE-KNGVDQETAARELLSEKQPS---LQFVTPEQLGGTAVFLASDAAAQITGTTVSVDGG------

General information: TITO was launched using: RESULT: Template: 2ZTV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1337 -26185 -19.58 -105.16 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -19.58 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.484

(partial model without unconserved sides chains): PDB file : Tito_2ZTV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZTV-query.scw PDB file : Tito_Scwrl_2ZTV.pdb: