Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKDPLTIIEQAEDHFIERIAENMHAFGMPSTVGRVLGIIYMNRKP-MTLTELSEATGMSKTRMSQVVREMLDANIAEKVFEKGVRKDLYEVEQDYYQTFITLFSATWSKVVSKNKMMHKKLNRELLSVLDEELTPEAEEKVNELLKELKEWLDYYNWLSRLIEFFESEDIFKYVPKP 4NB5 Chain:A ((15-159)) -----------DIMEFVEQMGGYFESRSLTRLAGRLLGWLLVCDPERQSSEELATALAASSGGISTNARMLIQFGFIERLAVAGDRRTYFRLRPN-----------AFAAGERERIRAMAELQDLADVGLRA-LGDAPPQRSRRLREMRDLLAYMENVVSDALGRYSQ----------

General information: TITO was launched using: RESULT: Template: 4NB5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 487 -15216 -31.24 -105.66 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -31.24 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.628

(partial model without unconserved sides chains): PDB file : Tito_4NB5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NB5-query.scw PDB file : Tito_Scwrl_4NB5.pdb: