Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIQNPYTAYQQNSVNTATPGELTLMLYNGCLKFIRLAAQAIEN----DDMERKNENLIKAQNIIQELNFTLNRN--IELSASMGAMYDYMYRRLVQANIKNDTGMLAEVEGYVTDFRDAWKQAIQSERKDRHGSGGIA 4IWB Chain:A ((43-164)) ----RGAEAYFQNQVETATPLEQIILLYDKAIECLERAIEIYDQVNELEKRKEFVENIDRVYDIISALKSFLDHEKGKEIAKNLDTIYTIILNTLVKV--DKTKEELQKILEILKDLREAWEEVKKKV-----------

General information: TITO was launched using: RESULT: Template: 4IWB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 318 -15271 -48.02 -131.65 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -48.02 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.523

(partial model without unconserved sides chains): PDB file : Tito_4IWB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IWB-query.scw PDB file : Tito_Scwrl_4IWB.pdb: