Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFIEQMFPFINESVRVHQLPEGGVLEIDYLRDNVSISDFEYLDLNKTAYELCMRMDGQKTAEQILAEQCAVYDESPEDHKDWYYDMLNMLQNKQVIQLGNRASRHTITTSGSNEFPMPLHATFELTHRCNLKCAHCYLESSPEAL---GTVSIEQFKKTADMLFD--NGVLTCEITGGEIFVHPNANEILDYVCKKFKK---------VAVLTNGTLMRKESLELLKTYKQKIIVGISLDSVNSEVHDSFR---GRKGSFAQTCKTIKLLSDHGIFVRVAMSVFEKNMWEIHDMAQKVRDLGAKAFSYN-WVDDFGRGRDIVHPTKDAEQHRKFM-----EYEQHVIDEFKDLIPIIP-----YERKRAANCGAGWK---SIVISPFGEVRPCALF-PKEFSLGNIFHDSYESIFNSPLVHKLWQAQAPRFSEHCMKDKCPFSGYCGGCYLKG--LNSNKYHRKNICSWAKNEQLEDVVQLI---------------------- 4K36 Chain:A ((2-392)) -----------PPLSLLIKPASS--------------------------------------------------------------------------------------------------------GCNLKCTYCFYHSLSDNVKSYGIMRDEVLESMVKRVLNEANGHCSFAFQGGEPTLAG--LEFFEKLMELQRKHNYKNLKIYNSLQTNGTLIDESWAKFLSE--NKFLVGLSMDG-PKEIHNLNRKDCCGLDTFSKVERAAELFKKYKVEFNILCVVTSNTARHVNKVYKYFKEKDFKFLQFINCLDPLYEEKGKYNYSLKPKDYTKFLKNLFDFWYEDFLNGNRVSIRYFDGLLETILLGKSSSCGMNGTCTCQFVVESDGSVYPCDFYVLDKWRLGNIQDMTMKELFETNKNHEFIKLS-FKVHEECKK--CKWFRLCKGGCRRCRDSKEDSALELNYYCQSYKEFFEYAFPRLINVANNIVDKLAAALEHHHHHH

General information: TITO was launched using: RESULT: Template: 4K36.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1634 53005 32.44 163.09 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 32.44 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.458

(partial model without unconserved sides chains): PDB file : Tito_4K36.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4K36-query.scw PDB file : Tito_Scwrl_4K36.pdb: