Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLTDKTVLITGGASGIGYAAVQAFLNQQANVVVADIDEAQGEAMIRKENNDRLH--FVHTDITDEPACQNAIRSAVDKFGGLDVLINNAGIEIVAP----IHEMELSNWNKVLNVNLTGMFLMSKHALKYMLKSGKGNIINTCSVGGVV--AWPDIPAYNASKGGVLQLTRSMAVDYAKHNIRVNCVCPGIIDTPLNEKSFLENNEGTLEEIKKEKAKVN-PLLRLGKPEEIANVMLFLASDLSSYMTGSAITADGGYTAQ 4WEC Chain:D ((27-272)) -RLAGKVAVITGGASGIGLATGRRLRAEGATVVVGDIDPTTGKAA-----ADELEGLFVPVDVSEQEAVDNLFDTAASTFGRVDIAFNNAGIS--PPEDDLIENTDLPAWQRVQDINLKSVYLSCRAALRHMVPAGKGSIINTASFVAVMGSATSQI-SYTASKGGVLAMSRELGVQYARQGIRVNALCPGPVNTPLLQELFAKDPE------RAARRLVHIPLGRFAEPEELAAAVAFLASDDASFITGSTFLVDGGISS-

General information: TITO was launched using: RESULT: Template: 4WEC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1299 -37331 -28.74 -157.51 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain D : 0.84 3D Compatibility (PKB) : -28.74 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.446

(partial model without unconserved sides chains): PDB file : Tito_4WEC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WEC-query.scw PDB file : Tito_Scwrl_4WEC.pdb: