Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTIFKQKNTHPFSNAANRLDRLPISRVHFQVLTALGIVYFFDLADLFTLSNVAPALIEHWGIPLSTIANVTAASFLGMFLGASLGGRLSDRIGRKKALNLFVFVFSIASLCNA------------------AAWDIPSLMTFRFLTGFGVAAAMVITNSYLAEFFPSSVRGKYISFCAMIGLIGVPITNIVSAFVIPLGS------WGWRLVFVWGAVGLIYFFFIHR-LEESPRWHENRGEYAKADAILTRI--EEQVEKEKGPLPAASQPKVSETVKQNAGYAGLLKGRNLKITIVLSAVWIFETFGFYGFASWVPSLLKSNGVTMENTLWYNVLHSVGAPLGALLGSMISERFQRKWILAASAFLTAIAGLLYGMTF------IPIMIIVFGFIVNITERVFTSNLYAYTSEPYPTEYRSSGSGLAYGLGRFSNIFGSLLVGF------IAVQLGYISVFLFIGGCWLACSLLLIFFGPNTNAKQI-------- 4GC0 Chain:A ((5-479)) ----------YNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVAPQNLS-----ESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGSAWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIRGKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLYTVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMF-----GVGVIVIGVMLSIFQQF--VGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIMTVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFA-MSWGPVCWVLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGCMGVLAALFMWKFVPETKGKTLEELEALWE

General information: TITO was launched using: RESULT: Template: 4GC0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2215 -243490 -109.93 -568.90 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -109.93 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.326

(partial model without unconserved sides chains): PDB file : Tito_4GC0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GC0-query.scw PDB file : Tito_Scwrl_4GC0.pdb: