Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLSELKTSGHPLTLLCSFLYFDVSFMIWVMLGALGVYISQD----FGLSPFEKGLVVAVPILSGSVFRIILGILTDRIGPKKTAVIGMLVTMIPLLWGTFGGRS-------LTELYAIGILLGVAGAS-FAVALPMASRWYPPHLQGLAMGIAG-AGNSGTLFATLFGPRLAEQ--------------FGWHIVMGIALIPLLIVFILFVSMAKDSPAQPSPQPLKSY----LHVFGQ----KETWFFCLLYSVTFGGFVGLSSFLSIFFVDQYQLSKIH----------AGDFVTLCVAAGSFFRPVGGLISDRVGGTKVLSVLFVIVALCMAGVSS-LPS--LSMVIVLLF-VGMMGLGMGNGAVFQLVPQRFRK-EIGMVTGIVGAAGGIGGFFLPNILGSLKQMTGTYAIGFITFSCIALLAFALVLAAGYYWRKSWSAESSPADV 4J05 Chain:A ((43-508)) --------------------GFFLDAYDLFIINQVAPMLAQVYFPKTGLPAQRQDLMKAAANIGCVVGQVMFGVLGDSFGRKFVYGKELILIIVATIFQMSA-PSHWDGNRVLTWITICRVFLGIGIGGDYPMSATVVSDRANIHRRGTLLCFIFANQGWGSFVGSLVTIVTISGFKHRLKSGHTHDVDKAWRILIGLSLIPAFGTLYQ-R------------------WQEFVAYFSTWNHFRNLLGSMLGWFLVDIAFYGINLNQSVVLAQ-IGFAGKTGDVYDKLFQLATGNIIVTALGFLPGYYFTLFLIDIVGRKKLQFMGFIMSGLFLAILAGEIDHIGKGPLLACFTFMQFFFNFGANTTTFIVAAELFPTRIRASAHGISAAAGKCGAILSSLVFNQLKAKIG-TSAVLWIFFSTCILGFISTFLIDETMGVD----------

General information: TITO was launched using: RESULT: Template: 4J05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1887 -304576 -161.41 -872.71 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -161.41 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.362

(partial model without unconserved sides chains): PDB file : Tito_4J05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J05-query.scw PDB file : Tito_Scwrl_4J05.pdb: