Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGFSHFNEQGRAQMVDISEKSSTVRTAAAVSSVHMKNEVYEKIQSHDIGKGDVLAVAQVAGIMAAKQTSNIIPMCHPLSLSGVDISFDWKIKEAEVILHIKAQVKTKGSTGVEMEALTSASVCALTVYDMCKALDKGMVIGPTFLLEKTGGKNGDFKRELSEYNLEDQK 2IDE Chain:D ((9-155)) ----------GRPRMVDVTEKPETFRTATAEAFVELTEEALSALEKGGVGKGDPLVVAQLAGILAAKKTADLIPLCHPLPLTGVEVRVE--LLKAEKRVRIEATVKTKAETGVEMEAMTACAVAALTVYDMLKAASKGLVISQVRLLHKAGGKSGEWRR-----------

General information: TITO was launched using: RESULT: Template: 2IDE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 747 -76153 -101.94 -518.04 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain D : 0.86 3D Compatibility (PKB) : -101.94 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_2IDE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IDE-query.scw PDB file : Tito_Scwrl_2IDE.pdb: