Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFFRNQLANVVEWEEFRDDMIFYKWNNREIKKGSRLIIRPGQDAVFLNNGR--VEGVFQDDGDYDIESEIIPFLSTLKGFKFGFNSGMRAEVLFVNTKEFTV--RWGTKQAINIPAAGMPGGMPIRANGTFNFKVQ-DYIS---LIDKIAGVKDQYFVEDIKTRIISILDQLLMKWITREGKDMFNLQANAFDIAKGIQED---------LDMQLISDGMTVTGFQIMSFNYPQEVQDMITKNASYGMVGDVNRYQQISMTDGMASGKMSGSGAASDMAGMMMGMNMANQMMNQMNQNQQAQSSGPQSTGSGSKPNFCPNCGTKTGEANFCPNCGQKLV 4HL8 Chain:A ((459-647)) -----------------------------------SYRVPHNAAVQVYDYRAKRARVVF-GPELVTL-----DPEEQFTVLSLSAGRPKRPHAR-RALC-LLLGPDFFT-DVITIETAD-HARLQL--QLAYNWHFELKNRNDPAEAAKLFS------VPDFVGDACKAIASRVRGAVASV--TFDDFHKNSARIIRMAVFGFEMSLPKARDQAVFPQNGLVVSSVDVQSVEPVD---------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4HL8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 709 -96532 -136.15 -592.22 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -136.15 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.318

(partial model without unconserved sides chains): PDB file : Tito_4HL8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HL8-query.scw PDB file : Tito_Scwrl_4HL8.pdb: