Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEETFQLLYDTYHQDLYQFLFYMVKDKNQTEDLLQEVYIRVLNSYHTFEGR-SSEKTWLLSIARHVAIDWFRKQQTIRQRILGTFDWDTQDVRDQQLLPDELAVQHENVREIYAAL-DQCTIDQRAVIILRFIQGYSIQETAKALRFSESKVKTTQHRGLKVLRKHMELLREELMDDEVRMERRTDKGVVKSTSGS 3MZY Chain:A ((45-200)) ------------------------------EDLVQEGILGLLKAIKFYDETKSSFSSFAFLCIRREMISAIRKANTQK---------------------EEAYLLKEEIEEFKKFSENNFSKFEKEVLT-YLIRGYSYREIATILSKNLKSIDNTIQRIRKKSEEWIKEEE-------------------------

General information: TITO was launched using: RESULT: Template: 3MZY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 401 13773 34.35 117.71 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 34.35 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.660

(partial model without unconserved sides chains): PDB file : Tito_3MZY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MZY-query.scw PDB file : Tito_Scwrl_3MZY.pdb: