Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVNEKRLLEEFLELVQIDSETKHEAEICKVLKRKFSDLGVDVKEDDTMD------I--------TGHGAGNLICTLKGTKQTDTIYFTSHMDTVVPGN--GV--KPV---VENGYVKTDGTTILGADDKAGLAAMFEAIKVLKEEN-IEHGTIEFIITVGEESGLIGAKALDRSMITASYGYALDSDGKVGNIIVAAPTQAKVRAAIFGKTAHAGVEPEKGISAITIASKAISKMPLGRID---------------EETTANIGRFEGGTQTNIVCDEVHILAEARSLVPEKMEAQVQKMKAAFEEAAADM----GGRAEVEIEV-MYPGFKYQDGDQVVEIAKKAAAK-IGRPSELQTSGGGSDANVIA-GHGIPTVNLAVGYEQIHTKNEKMPIEELVKTAEMVVAIIEEAAK 3PFO Chain:A ((24-428)) --NFNDQVAFLQRMVQFRSVRGEEAPQQEWLAQQFADRGYKVDTFSLADVDIASHPKAAPMDTIDPAGSMQVVATADSDGKGRSLILQGHIDVVPEGPVDLWSDPPYEAKVRDGWMIGRGA----QDMKGGVSAMIFALDAIRTAGYAPDARVHVQTVTEEESTGNGALSTLMRGYRADACLIPEPTG--HTLTRAQVGAVWFRLRVRGTPVHVA-YSETGTSAILSAMHLIRAFEEYTKELNAQAVRDPWFGQVKNPIKFNVGIIKGGDWASSTAAWCELDCRLGLLTGDTPQEAMRGIEKCLADAQATDSFLSENPAELVWSGFQADPAVCEPGGVAEDVLTAAHKAAFNAPLDARLSTAVNDTRYYSVDYGIPALCYGPYGQGPHAFDERIDLESLRKTTLSIALFVAEWC-

General information: TITO was launched using: RESULT: Template: 3PFO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1901 1712 0.90 4.74 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 0.90 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_3PFO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PFO-query.scw PDB file : Tito_Scwrl_3PFO.pdb: