Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MRNKRRNRQIVVAVNGGKAVKAIFLFI-------VSLIVIFVLSGVLTSL--RP-ELRPSSDSFYGIAEELPGD-------VFAHLLQMENHYFASDLSQTDSSFHLSRLSLKLATSINLEDPRSFLGRELPGFAQFDTEILLAGQGTDYTNMPAESPPPSKVMEEEREANLAEIEKQQTQSDNAQKDP--------PKQTTGDKKVVFIYHTHNTESYLPLLKGETDPDMARH---------------SKANVTL--VGDMFGQALESQGIGATVNKTDIQSKLNKKGLNYARSYDESRPVVKD----ALASNKNLQYIIDIHRDSRRKKDTTATIKGKSYARVAFVVGKKSKNFEENYKIASELHKLMEKKYPGLSTGVFSKGSPGDNGVYNQD-----LTDRALLLEF------GGVDNNLEELQ----RAANAAADVFSEMYW-----DAEKVNAASGETKKQ- 5DA0 Chain:A ((14-484)) QREWFANPRKDVLAGIVVALALIPEAIAFSIIAGVDPQVGLYASFIIALITAFLGGRPGMISAATGAMALLMTGLVKDHGIQYLFAATVLTGVLQVVFGWAKLARYLKFVPRSVMVGFVNALAILIFMAQLPQFVGANWQMYAMVAAGLAIIYLLPLVFKAMPSALVAIVVLTVVAVVTGADVKTVGDMGTLPTALPHFQFPQVPLTFETLAIIFPVALTLSLVGLLESLLTAQLIDERTDTTSDKNVESRGQGVANIVTGFFGGMAGCAMIGQSMINVTSGGRGRLSTFVAGAFLMVLILALQPLLVQIPMAALVAVMMVVAISTFDWGSLRFPKGETVVMLATVAVTVFT-----HDLSLGVLIGVVLSALFFARKVSQLSQVTPVDEVDGTRTYRVRGQLFFVSTHDFLHQFDFTHPARRVVIDLSDAHFWDGSAVGALDKVMLKFMRQGTSVELRGLNAASATLVERL

General information: TITO was launched using: RESULT: Template: 5DA0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2116 -130963 -61.89 -330.71 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -61.89 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.194

(partial model without unconserved sides chains): PDB file : Tito_5DA0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DA0-query.scw PDB file : Tito_Scwrl_5DA0.pdb: