Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEPLKSHTGKAAVLNRINVDTDQIIPKQFLK-RIERT----GYGRFAFFDWRYDANGEPNPEFELNQP--------VYQGASILIAGENFGCGSSREHAPWA------------LDDYGFKIIIAPSFADIFHQNCFKNGMLPIRMPYDNWKQLVGQYENQSLQMTVDLENQLIHD-SEGNQIS-F-EVDPHWKEMLINGYDEISLTLLLEDEIKQFESQRSSWLQA 1L5J Chain:A ((164-343)) ----EKLTVTVFKV-TGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIE--------PDQPGVVGPIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGGKIAPIFFNTMEDAGALPIEVDV---S----NL-NMGDVIDVYPYKGEVRNHETGELLATFELKTDVLIDEVRAGG--------------------------

General information: TITO was launched using: RESULT: Template: 1L5J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 777 -209 -0.27 -1.38 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -0.27 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.356

(partial model without unconserved sides chains): PDB file : Tito_1L5J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1L5J-query.scw PDB file : Tito_Scwrl_1L5J.pdb: