Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIKTISGAERLSLKRSIDAGTEEQRKTVRSIIEDVKANGDQAVRSYTAKFDCIEID------SP-LVTKEE--FEEAYTSLDSRLLQVIRQA-IENIREYHERQLQSSWFYHRKDGTMLGQKVTALDSAGVYV--PGGTAAYPSSV-LMN-------VIPALVAGVE----RIVLVTPPGKDGLLSPGVLVAAAEL-----GIKDIYKMGGAQAIAALAYGTETIEPVDKITGPGNIYVALAKREVFGDVDIDMIAGPSEIVVLADETAIPSEIAADLLSQA-EHDKLSSCVFVT-DSMALAETVSAEVNKQLETLPRREIAEASVRDYGCIYVAETMVEAIETVNTLAPEHLEI-ITQSPEALLGSIKHAGAIFLGRYSPEPVGDYFAGPNHVLPTNGTARFSSPLNVTDFQKKSS-I-ISYSQSAFEEHAESIAAFARLEGLEAHARSIEARERRISK- 4MTN Chain:A ((5-406)) ----------GNEVLRIVDSIHRDKSIDKEIVFEGVEQAILSAARKHFGEEEVIEVHIDRTSGQPMVKTNGREIDRDELGDILGRISAQTKQVMIQKIREAERDTLFDE--YAQLRGQIVSGTVTRNEGSAITVNIGKAEAILPRSEMIPGESHRPNERIRAVVLEVKKMGPRVRVVLSRAHPDFVRRLLELEIPEVNERIIEIRSLAREAGYRTKVAVSCADSNIDPVGACVGVRGARIRNVGEELGGE-RIEVVRWNDS--------------LQVLVPNAMQPSEVEDVILCPML-------------GRVLVLVRDDQLSLAIGKRG---------QNVRLASKLVGWDIDVMTREELDQQLDQAVVAYSQIPG--VSEELAEGLVSQG----------FLSFEDLSVIEPDELMEMGSLTQEQADVIVEYAERESERIEKEQDLRRATEKAERQSQE

General information: TITO was launched using: RESULT: Template: 4MTN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1625 70636 43.47 192.99 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 43.47 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.286

(partial model without unconserved sides chains): PDB file : Tito_4MTN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MTN-query.scw PDB file : Tito_Scwrl_4MTN.pdb: